I wasn't a DC fan when I collected, "Make mine Marvel", all of my knowledge of DC came from the various cartoon series going back to Superfriends and through all of the WB DC animated series. And if I was curious about some character I hadn't heard of before or thought was interesting I would look it up on Wikipedia.
So I do get the implied reference calling his sister "Speedy". I recognized the Royal Flush gang, even though they were missing 10. I realized quickly that they were doing the origin of "The Huntress", well one of them. I recognized Deathstroke's mask (though I first knew him as Slade on Teen Titans). "Bullseye" goes without saying since that was his Interpol nickname in the episode but most times they don't identify the various signature DC villains by their comic book name.